Fig. 3
Defensive responses of rice plants in response to oral secretion (OS) collected from Chilo suppressalis (SSB) larvae with or without AB treatment. Rice stem samples were harvested 48 h after mechanical wounding with application of OS + AB and OS-AB, and the Lipoxygenase (LOX) activity (A) and Trypsin protease inhibitor (PI) content (B) were measured (LOX, F(3,36) = 40.39, P < 0.001; PI, F(3,36) = 106.5, P < 0.001; n = 10). CK, untreated rice plants; Water, rice stems wounded and applied with sterilized water; OS-AB, rice stems wounded and treated with oral secretion collected from larvae without AB; OS + AB, rice stems wounded and treated with oral secretion collected from larvae with AB. C The 3rd instar larvae were allowed to feed on rice plants pre-treated by mechanical wounding with application of OS + AB and OS-AB for 3 days, and then larval mass gain was determined (F(3,36) = 106.5, P < 0.001; n = 10). CK, larvae fed on untreated rice plants. Values are means ± SEM. Different letters indicate statistically significant differences between treatments based on an ANOVA followed by LSD test (P < 0.05)