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Fig. 8 | Rice

Fig. 8

From: Iron Induces Resistance Against the Rice Blast Fungus Magnaporthe oryzae Through Potentiation of Immune Responses

Fig. 8

Accumulation of ROS and Fe in leaves of Control and High-Fe rice plants. A ROS (H2O2) was detected in leaves using the fluorescent probe H2DCFDA. Leaves (youngest developed leaves) from mock-inoculated (mock) and M. oryzae-inoculated plants (48 hpi) were examined. Bars correspond to 2 mm. Right panel, quantification of ROS fluorescence using Image J software (three independent experiments, 100 fields, each). Asterisks indicate statistically significant differences calculated by two-way ANOVA (*P ≤ 0.05). B Fe detection in leaves of Control-Fe (upper panels) and High-Fe (lower panels) plants that have been mock-inoculated or infected with M. oryzae (48 hpi). The youngest developed leaf (third leaf) was subjected to DAB staining (brown) followed by Perls staining (blue). Bars correspond to 50 μm. St, stomata, Ap: appressorium

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