Fig. 7
Effect of pathogen infection on Fe homeostasis-related genes and pathogen-induced alterations on Fe content in leaves. A Heat map showing the expression level of iron-related genes (row scaled FPKMs) in leaves of Control and High-Fe plants (mock-inoculated and M. oryzae-inoculated) at 48 hpi. Gene expression is represented from pale yellow (less expressed) to brown (more expressed). The full name and details on the expression of these genes are indicated in Additional file 7: Table S6. B Total Fe content in the youngest developed leaf (third leaf) (−, mock-inoculated; +, M. oryzae-inoculated). Five independent biological replicates (three technical replicate each) were analyzed. C Fe2+ and Fe3+ content was determined by the colorimetric Ferrozine method in the youngest developed leaf of Control and High-Fe plants. D Fe content in the apoplast of leaves (youngest developed leaves) determined by ICP-MS. Five independent biological replicates (three technical replicate each) were analyzed. Data are mean ± SEM. Asterisks indicate statistically significant differences calculated by two-way ANOVA *, P ≤ 0.05). DW, dry weight; FW, fresh weight