Fig. 5

Expression of genes involved in defense responses in leaves of Control and High-Fe plants. Heat maps (A and B) show the expression pattern of DEGs identified by RNA-Seq analysis (Mock and infection conditions, 48 hpi) as row scaled FPKM (fragments per kilobase per million reads), from pale yellow (less expressed) to brown (more expressed). A Expression of Pathogenesis-Related (PR) genes. B Expression of lipoxygenase and phenylpropanoid genes. Gene names, locus IDs, FPKMs and fold change values are indicated in Additional file 5: Table S4. (C) Transcript levels were determined by RT-qPCR analysis (−, mock-inoculated; +, M. oryzae-inoculated). The expression values were normalized to the rice Ubiquitin1 gene. Four independent biological replicates were examined. Data are mean ± SEM. Asterisks indicate statistical significance calculated by two-way ANOVA (*, P < 0.05; **, P < 0.01, *** P < 0.001). Gene-specific primers are listed in Additional file 9: Table S8