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Fig. 6 | Rice

Fig. 6

From: The PPR-Domain Protein SOAR1 Regulates Salt Tolerance in Rice

Fig. 6

Performance of OsSOAR1-transgenic plants under salt stress at early seedling stage. A Phylogenic analysis of AtSOAR1 with the homozygous PPR genes in rice using the neighbor-joining method with MEGA version 11 by alignment of the gene sequences with ClustalW. B Real-time PCR detection of OsSOAR1 transcripts level in wild-type and the two transgenic lines OsSOAR1-OE1 and OsSOAR1-OE2. Total RNA was extracted from two-week-old seedlings and used for cDNA synthesis. Actin1 was used for internal control and the expression level of OsSOAR1 in WT was taken as 1. All the values are means ± SE from three independent biological determinations. C Phenotypic comparison of wild-type and OsSOAR1-transgenic plants OsSOAR1-OE1 and OsSOAR1-OE2 grown under 140 mM NaCl solution at the seedling stage. Hydroponic cultured two-week-old seedlings were treated with 140 mM NaCl for 8d, and then recovered for 7d. Three independent biological determinations were conducted and similar results were obtained. D Survival rates of the wild-type and transgenic plants as described in C after 7d recovery. All the values are means ± SE from three independent biological determinations. Student’s t test was used for comparing the survival rate of each overexpression line with those of wild-type plants (with significant differences at **P < 0.01). E, F Chlorophyll content E and relative ion leakage F of the wild-type and transgenic plants under normal and salt stress conditions. Two-week-old seedlings were treated with 100 mM NaCl for 9 d to assay chlorophyll content, or for 24 h to detect relative ion leakage. Student’s t test was used for comparing the chlorophyll content or relative ion leakage of each overexpression line with those of wild-type plants (with significant differences at **P < 0.01)

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