Fig. 3
From: The PPR-Domain Protein SOAR1 Regulates Salt Tolerance in Rice
Phenotypes of AtSOAR1-transgenic plants in ABA-induced early seedling growth arrest. A Phenotypic comparison of wild-type and AtSOAR1-transgenic plants OE-1 and OE-2 in ABA-induced inhibition of seedling growth. Germinated seeds of different genotypes were cultured in ABA-free (0 μM) or ( ±) ABA-containing (4 and 8 μM) solutions for 9 d before investigation. The experiments were repeated for three times and similar results were obtained. B Quantitative evaluation of shoot and root length of the wild-type and transgenic plants as described in A. All the values are means ± SE from three independent biological determinations. Student’s t test was used for comparing the shoot length or root length of each overexpression line with those of wild-type plants (with significant differences at **P < 0.01). C Detection of ABA-responsive genes in wild-type and AtSOAR1-transgenic lines by qPCR and semi-quantitative RT-PCR. The bands from left to right indicates WT without treatment, OE-1 without treatment, OE-2 without treatment, ABA-treated WT, ABA-treated OE-1 and ABA-treated OE-2, respectively. Materials described in A was sampled for total RNA extraction and cDNA synthesis. Actin1 was used for internal control and all the values are means ± SE from three independent biological determinations