Targeted Identification of Rice Grain-Associated Gene Allelic Variation Through Mutation Induction, Targeted Sequencing, and Whole Genome Sequencing Combined with a Mixed-Samples Strategy

Rice

Table 3 Amino acid mutation and phenotypic information of single mutant plants

Mutant number	Single plant number	Amino acid mutation	Grain length (mm)	Grain width (mm)
WT	WT		9.31	2.03
GS3-1	7-112-2-4	A5G	9.21	2.12
GS3-2	4-101-1-3	C55X	10.22**	2.03
GS3-3	5-6-1-4	C135W	8.06**	2.04
GS3-4	4-101-2-2	S141C	9.41	2.17
GS3-5	6-23-1-4	C173F	8.51**	1.9
GS3-6	7-78-1-3	G167C	9.33	1.94
GS3-7	9-5-1-1	C190F	9.97**	1.86
GS3-8	7-78-2-3	S184F	9.33	2.09
GS3-9	7-41-1-4	R200H	9.30	1.9
GW5-1	5-35-1-2	V411G	9.53	2.29**
GW5-2	7-82-1-4	A405G	9.42	2.02
GW5-3	10-14-2-1	K397E	9.46	2.03
GW5-4	10-51-2-3	D97G	9.40	1.84**

*: represents significant variation at p < 0.05; **: represents highly significant variation at p < 0.01