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Rice
Fig. 1 | Rice

Fig. 1

From: Targeted Identification of Rice Grain-Associated Gene Allelic Variation Through Mutation Induction, Targeted Sequencing, and Whole Genome Sequencing Combined with a Mixed-Samples Strategy

Fig. 1

Simplified steps of the MTWA process. a Wild-type (WT) seeds were irradiated with a carbon ion beam, and the M2 generation was planted and harvested. b The M2 generation mixed samples were constructed at a ratio of 8:1 for targeted sequencing detection, and the grain shape of the M2 generation seeds was measured. c Sanger sequencing was performed on individual plants in a mixed sample of potential mutations detected by targeted sequencing and compared with phenotypes to identify key mutants. d WGS of key mutants to resolve associations between mutant phenotypes and genotypes and to mine for novel allelic variants

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