Fig. 2

WG3 interacts with DLT to regulate grain size and BR signaling in rice. (A) BiFC analysis of WG3 and DLT interaction in the epidermal cells of Nicotiana benthamiana leaves. Scale bar, 50 μm. (B) Plant architecture of R498, wg3, dlt, and wg3/dlt at the mature stage. Scale bar, 10 cm. (C) Comparison of grain width between R498, wg3, dlt, and wg3/dlt. Scale bar, 3 mm. (D-F) Statistical analysis of grain length (D), grain width (E), and 1000-grain weight (F) between R498, wg3, dlt, and wg3/dlt. Data are means ± SD (n = 3 replicates). Significant differences between R498, wg3, dlt, and wg3/dlt were determined using Duncan’s multiple comparisons. (G) Lamina inclination of R498 and wg3 plants treated with different concentrations of 24-epiBL. Scale bar, 1 cm. (H) Statistical analysis of lamina joint angle in G. Data are means ± SD (n = 20 plants). (I) Comparison of expression levels of four BR biosynthesis genes (OsDWARF, OsDWARF4, D2, and D11) in the young panicles of R498 and wg3. UBQ5 and FhaB were used as internal controls, and the values of R498 were set to 1. Data are means ± SD (n = 3 replicates). Significant differences between R498 and wg3 (**P < 0.01) were determined using Student’s t-test