Fig. 1

Loss of OsPHYB function resultes in larger grain size and increases chalkiness in rice. A Location of OsPHYB knockout target sequence. B The sequencing results of target sequence for three T₃ generation pure and knockout lines of OsPHYB. C–D Grain size comparison of osphyb mutant and NIP plants in rice and brown rice. Bars = 5 mm. E–I Statistics of grain length, grain width, grain thickness 1000-grain weigh and brown rice 1000-grain weight of osphyb mutant and NIP rice in 2019 and 2020. P < 0.05, ** P < 0.01. J–L Statistics of osphyb mutant and NIP grain chalkiness rate, whiteness index and head rice rate in 2019 and 2020. *P < 0.05, ** P < 0.01