Fig. 8

PCR validation of RNA-Seq data. Several differential expressed genes detected in RNA-Seq data were used to do qRT-PCR validation. A and B. Two salt-induced genes were expressed at higher levels in ‘C34’. C. A lncRNA induced significant induced in ‘C34’ by salt stress. D. A salt-suppressed gene show less expression in ‘C34’ when compared with ‘NB’ and ‘IR29’. E–G. RT-PCR test pre-mRNA alternative splicing events. Schematic diagram generated from comparison of genome sequence, supposed reference mRNA sequence and sequencing results in SnapGene program is shown below each RT-PCR gel electrophoresis. The AS type of PCR amplified fragment was marked by arrows with legend. CS: Constitutive splicing; ES: Exon skipping; IR: Intron retention; 3’SS: Alternative 3’ splice site; 5’SS: Alternative 5’ splice site; “*” means AS type needs further determined. E. RT-PCR validation of alternative splicing of coding genes. F. RT-PCR validation of alternative splicing of lncRNAs. G. RT-PCR test pre-mRNA splicing patterns of SR genes