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Rice
Fig. 4 | Rice

Fig. 4

From: Simultaneous Enhancement of iron Deficiency Tolerance and Iron Accumulation in Rice by Combining the Knockdown of OsHRZ Ubiquitin Ligases with the Introduction of Engineered Ferric-chelate Reductase

Fig. 4

Features of the HRZ2i + Refre lines compared with single transformation lines in non-submerged calcareous soil. A SPAD value of the newest leaves. Data for NT on day 0 were around 30 but not recorded; data for NT on days 35 and 37 were unavailable due to the death of all new leaves. B Shoot height. C Representative plants on the 59th day after transplanting. Scale bar = 10 cm. D Panicle and straw dry weights after seed maturation. All the surviving plants were collected. An “X” mark indicates no yield. E Fe concentrations in the brown seeds and straw following seed maturation. The following lines were cultivated: NT, non-transformants; HRZ2i + Refre, T1 lines of 1 and 2; HRZ2i, T2 line 3; Refre, T3 line 7. DW: dry weight. For A and B, means ± SE are shown (n = 10 for HRZ2i + Refre line 2; n = 5 for other lines). For E, means ± SD are shown (n = 6 for HRZ2i + Refre line 2 for seed Fe; n = 3 for Refre line 7 for seed Fe; n = 7 for HRZ2i + Refre line 2 for straw Fe; n = 4 for other lines for straw Fe). Different letters indicate significant differences (P < 0.05; Tukey’s honest significant difference test). Datum for NT on day 32 was not used for this test due to the death of all new leaves except for one line

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