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Fig. 2 | Rice

Fig. 2

From: OsWHY1 Interacts with OsTRX z and is Essential for Early Chloroplast Development in Rice

Fig. 2

Disruption of OsWHY1 function causes an albino-lethal phenotype at the seedling stage. a Schematic diagram showing the target site of OsWHY1 by the CRISPR/Cas9 system. The target sequences within the first exon of OsWHY1 are marked in green. b Phenotypes of the wild-type (WT) and the oswhy1 mutant at the two-leaf stage. Seedlings were grown in a growth chamber with 14 h of light (300 μmol photons m−2 s−1) and 10 h of darkness at constant temperatures of 30 °C. Bar = 3 cm. c Two modes of mutation in the oswhy1 mutants. The oswhy1-1 mutant is an insertion mutation, and the oswhy1-2 is a deletion mutation. d Relative OsWHY1 transcript levels in the WT and oswhy1 plants. The OsWHY1 transcript levels in the WT were set to 1. e The contents of chlorophyll a (Chl a), chlorophyll b (Chl b), and carotenoid (Car) in the WT and oswhy1-1 plants at the two-leaf stage. f Relative OsTRX z transcript levels in the WT and oswhy1-1. Data are the mean ± SD (n = 3). **p < 0.01 (Student’s t-test)

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