Fig. 5
From: OsFTL4, an FT-like Gene, Regulates Flowering Time and Drought Tolerance in Rice (Oryza sativa L.)
Interactions among OsFTL4, 14-3-3, and OsFD1 proteins. a Y2H assay. OsFTL4 was used as bait (GAL4-BD, BD), whereas GF14a to GF14h and OsFD1 were used as prey (GAL4-AD, AD). DDO: SD/-L/-T, QDO: SD/-L/-T/-H/-A. The 3-AT concentration was 25 mM. The interaction between OsFTL4 and GF14a b, GF14b c, GF14c d, GF14d e, GF14e f, GF14f g, GF14g h, GF14h i, or OsFD1 j was determined by the LCI assay involving N. benthamiana leaf epidermal cells. Effect of OsFTL4 on the activation of OsMADS15 expression by FAC. Data are presented as the mean ± SEM of three independent experiments