Fig. 2
From: OsFTL4, an FT-like Gene, Regulates Flowering Time and Drought Tolerance in Rice (Oryza sativa L.)
Knockout of OsFTL4 affects the heading date and other agronomic traits in rice. a A specific target guide RNA located in the first exon was used to edit OsFTL4 via the CRISPR/Cas9 system. The PAM region and guide sequence are marked in red and blue, respectively. The OsFTL4 mutation sites in the two mutants (osftl4-1 and osftl4-2) are indicated. b Mutations were confirmed by sequencing. Mutated bases are marked with red boxes. c OsFTL4 protein structures in GLA 4, osftl4-1, and osftl4-2. The PEBP domain is marked in yellow. d Morphology of GLA 4, osftl4-1, and osftl4-2 plants at heading stage. Scale bars, 10 cm. e Panicle phenotype of GLA 4, osftl4-1, and osftl4-2. f Morphology of GLA 4, osftl4-1, and osftl4-2 plants at maturity satge. Scale bars, 10 cm. g Panicle and internode at maturity. Scale bars, 10 cm. h Length of four internodes below the spike. Heading date for GLA 4, osftl4-1, and osftl4-2 in Lingshui j and Yangzhou k. Comparison between GLA 4 and the mutants in terms of the plant height i, panicle length l, panicle number per plant m, grain number per panicle n, 1,000-grain weight o, and grain yield per plant p. Data are provided as the mean ± SD from 20 individual plants. Significant differences were determined according to Student’s t-test (**, p < 0.01)