Fig. 6
From: Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice
OsSKL2 interacts with OsASR1. A OsSKL2 was found to interact with OsASR1 in a yeast two-hybrid system, using BD-OsSKL2 + AD, AD-OsASR1 + BD as negative controls. Growth on selective QDO plates indicated a positive interaction. DDO: yeast SD/-Trp/-Leu selective medium, QDO: yeast SD/-Trp/-Leu/-His/-Ade selective medium. B A pull-down assay was then performed to confirm the interaction between OsSKL2 and OsASR1 in vivo. GST and GST-OsASE1 proteins coupled to beads were incubated with MBP-OsSKL2 fusion proteins. Bound OsSKL2 was then detected using mouse anti-MBP antibody. C A BIFC assay further revealed that OsSKL2 interacts with OsASR1 in rice leaf protoplasts (bar = 10 μm)