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Fig. 9 | Rice

Fig. 9

From: Rice MPK17 Plays a Negative Role in the Xa21-Mediated Resistance Against Xanthomonas oryzae pv. oryzae

Fig. 9

Expression of WRKY in MPK17-regulated transgenic rice after inoculated with Xoo. A Expression of five WRKY proteins in MPK17-RNAi transgenic rice after inoculated with Xoo. B Expression of five WRKY proteins in MPK17-OX transgenic rice after inoculated with Xoo. C Quantitative comparison of WB intensities of WRKY62 protein in panel (A). D Quantitative comparison of WB intensities of WRKY62 protein in panel (B). WRKY42: anti-WRKY42 antibody-detected band; WRKY62: anti-WRKY62 antibody-detected band; WRKY67: anti-WRKY67 antibody-detected band; WRKY68: anti-WRKY68 antibody-detected band; WRKY76: anti-WRKY76 antibody-detected band; HSP82: anti-HSP82 antibody-detected band used as loading control. i118, i120: independent MPK17-RNAi transgenic lines; OX202, OX208: independent MPK17-OX transgenic lines; TP309: Japonica rice variety; 4021: transgenic TP309 line harboring the Xa21 gene. Signal of WRKY62 protein in panel A and B were detected by Mini Chemiluminescent Imager and Sage Capture software. Lane 1D software was used to extract signals of WB. Average was calculated for three repeats. Error bars are standard deviation (SD). **designate difference at p < 0.01 level

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