Fig. 8

Abundance of Xoo proteins in rice expressing different levels of MPK17 following Xoo inoculation. A MPK17-RNAi transgenic plants. B MPK17-OX transgenic plants. Inoculated rice plants were cultivated at 31℃ (MPK17-RNAi transgenic rice) or 27℃ (MPK17-OX transgenic rice). The whole leaves were collected at 0, 6, 9 dpi, total proteins were isolated and separated by SDS-PAGE, then detected by anti-Xoo antibody (Additional file 1: Fig. S2). Protein signal were detected by Mini Chemiluminescent Imager and Sage Capture software. Lane 1D software was used to capture the signals of WB. Mean was calculated for three repeats. Error bars are standard deviation (SD). **denotes difference at p < 0.01 level. i118, i120: independent MPK17-RNAi transgenic lines; OX202, OX208: independent MPK17-OX transgenic lines; TP309: Japonica rice variety; 4021: homozygous transgenic TP309 line harboring the Xa21 gene