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Fig. 2 | Rice

Fig. 2

From: Rice MPK17 Plays a Negative Role in the Xa21-Mediated Resistance Against Xanthomonas oryzae pv. oryzae

Fig. 2

Identification of MPK17-RNAi transgenic lines by PCR and WB. i118, i120: independent MPK17-RNAi transgenic lines; CK: 4021 (homozygous TP309 transgenic line harboring the Xa21 gene); 1–8: individual transgenic plants in T3 generation; PCR: PCR genotyping results done with primers (5’-GAGTCGTAAGAGACTCTGTATG-3’ and 5’- GCGAGCTCGGTTTTCAGTTGAGCAAC-3’). MPK17: anti-MPK17 antibody-detected band; HSP82: anti-HSP82 antibody-detected band used as loading control. PCR: PCR product separated on agarose gel. WB: detection of MPK17 using anti-MPK17 antibody

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