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Fig. 5 | Rice

Fig. 5

From: OsSNDP3 Functions for the Polar Tip Growth in Rice Pollen Together with OsSNDP2, a Paralog of OsSNDP3

Fig. 5

Double mutant phenotype of ossndp2 ossndp3. The expression pattern of OsSNDP1 (a) and OsSNDP2 (b and c). d Sequencing analysis of the target region of CRISPR/Cas9 on OsSNDP2 and OsSNDP3 from double mutants. Red characters indicate inserted sequences by the gene-editing system and red dashes indicate deleted sequences. In vitro pollen germination from wide type (e and g) and ossndp2 ossndp3 (f and h). Pictures were taken 30 min after germination on the solid germination media. Arrow indicated long pollen tube from wild type and triangle marked protrusion of pollen tube. Magnified images of the red box were shown in g and h. Representative images were taken from ossndp2 ossndp3-1. i pollen tube length from wild type and ossndp2 ossndp3. Values are means (± SE) of pollen tube lengths (n > 20 pollen tubes)

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