Fig. 3

The mutant phenotype of OsSNDP3. a Sequencing analysis of the target sites of CRISPR/Cas9 on the genomic regions of OsSNDP3. Red characters indicate mutated sequences. b Fertilities of nine panicles from wild type and ossndp3. Each three panicles used from ossndp3-3, ossndp3-4 and ossndp3-5. c Picture of wild type, ossndp3-3 and ossndp3-9 at ripening stage. Flower structure (d and e), pollen in PBS (f and g) and pollen stained by Lugol’s iodine (h and i), and Auramine O (j and k) from the WT (d, f, h, and j) and ossndp3-3 (e, g, i, and k). In vitro pollen germination of wide type (l) and ossndp3-3 (m). Pictures were taken 30 min after germination on solid germination media. Arrow represented long pollen tube from wild type and triangle indicated short pollen tube from ossndp3-3. n pollen tube length from wild type and ossndp3-3. Values are means (± SE) of pollen tube lengths (n > 25 pollen tubes). In vivo pollen germination stained by aniline blue (o–t). The pollens on stigma germinate through the style and enter the micropyle (open circle) in wild type (o, q, and s). The short pollen tube (triangle) stayed in the stigma of ossndp3-3 (p, r, and t). Bars = 1 mm (d and e), 50 µm (f–m), 500 µm (o and p), and 100 µm (q–t)