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Fig. 4 | Rice

Fig. 4

From: Magnaporthe oryzae Chloroplast Targeting Endo-β-1,4-Xylanase I MoXYL1A Regulates Conidiation, Appressorium Maturation and Virulence of the Rice Blast Fungus

Fig. 4

Subcellular localization of the relative expression xylanases at different stages of M. oryzae -host interaction. a Localization of MoXYL1A in the aberant conidia, conidia germination and appressorium formation stages of M. oryzae was determined by transforming a MoXYL1A-GFP fusion construct into the protoplast of the wild type strain Guy11 and examining fungal cells using the Scale bar = 20 µm. DIC indicates bright field illumination. GFP was excited at 488 nm. b Localization of MoXYL1B was assessed as in (a). MoXYL1B-GFP signal is evident in the conidium and appressorium. Scale bar = 20 µm. c In-planta expression of MoXYL1A and MoXYL1B transcripts during distinct stages of host–pathogen interaction was assessed by qRT-PCR. Vegetative hyphae were used as a control stage and the expression level of MoXYL1A and MoXYL1B at the hyphal stage was set to 1. Error bars represent standard deviation (SD). SD was calculated from three independent biological replicates along with three technical replicates. (*, P < 0.05 by t-test)

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