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Fig. 3 | Rice

Fig. 3

From: Magnaporthe oryzae Chloroplast Targeting Endo-β-1,4-Xylanase I MoXYL1A Regulates Conidiation, Appressorium Maturation and Virulence of the Rice Blast Fungus

Fig. 3

MoXYL1A and MoXYL1B mutants show varying degrees of sensitivity to cell wall perturbing agents. a Physical inhibitory effect of selected cell wall stress-inducing agents on the vegetative growth of the individual strains. The strain were cultured on CM media supplemented with 200 μg/mL Calcofluor White (CFW), 0.01% SDS or 200 μg/mL Congo Red (CR) for 10-days. b Quantification and statistical evaluation of the response of MoXYL1 single and double deletion mutants and the wild-type strain to different cell wall stress inducing reagents. The inhibition data was generated from five independent biological experiments with five technical replicates each. One-way ANOVA (non-parametric) statistical analysis was carried out with GraphPad Prism8 and Microsoft Excel. Error bars represent standard deviation. Inhibition rate was calculated as a percentage = (the diameter of control − the diameter of treatment)/ (the diameter of control) × 100. Single asterisk represents a significant difference (p < 0.05)

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