Fig. 6
From: Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice
Functional characterization in yeast. A GFP-COT1 and GFP-OsFPN1 localization in the cot1 yeast mutant. Bar = 10 µm. B High Co tolerance assay of yeast used in A. Yeast cells were adjusted to the indicated OD and spotted on SD-uracil medium containing 0 or 1 mM of Co. Images were captured after 2 d of incubation. C Co and Ni concentration in cot1 expressing GFP-COT1 and GFP-OsFPN1. Yeasts were incubated with 50 µM of Co or Ni for 1 h, and Co and Ni were determined by ICP-MS. n = 3–6. Dunnett’s test compared to the vector, **p < 0.01