Development of a Temperate Climate-Adapted indica Multi-stress Tolerant Rice Variety by Pyramiding Quantitative Trait Loci

Rice

Table 1 Yield and yield-related traits of CSA-Pi9 and CSA plants grown under various paddy field conditions

Field location¹	Variety	Generation	Growth period (DAT)²	Flowering period (DAT)²	Culm length (cm)³	Panicle length (cm)³	Panicle number³	Spikelet number³	Fertility (%)³	Hundred-grain weight (g)³	Grain yield⁴
Field A (2018)	CSA	–	146	64	90.67 ± 0.67	27.50 ± 0.50	9.67 ± 0.33	145.78 ± 6.41	89.03 ± 0.93	2.56 ± 0.03	25.49 ± 2.03
	CSA-Pi9	BC₂F₅	146	64	81.00 ± 1.73^*	27.17 ± 0.60	11.00 ± 1.53	187.22 ± 9.02^**	90.15 ± 1.34^***	1.99 ± 0.01^***	36.40 ± 0.53^*
Field A (2019)	CSA	–	135	91	75.45 ± 0.57	25.02 ± 0.50	9.72 ± 0.47	141.56 ± 3.35	95.40 ± 0.76	2.66 ± 0.01	30.75 ± 1.22
	CSA-Pi9	BC₂F₇	135	76	66.83 ± 1.20^***	25.40 ± 0.37	8.50 ± 0.47	182.67 ± 2.71^***	93.64 ± 0.55	2.58 ± 0.01^**	27.95 ± 1.43
Field B	CSA	–	128	92	73.40 ± 1.21	23.67 ± 1.20	15.33 ± 0.33	129.13 ± 3.11	65.95 ± 1.80	2.34 ± 0.02	32.63 ± 3.70
	CSA-Pi9	BC₂F₇	128	77	69.57 ± 1.07^**	22.83 ± 0.48	14.67 ± 0.88	122.75 ± 5.39	93.29 ± 1.73^***	2.19 ± 0.03^*	32.00 ± 3.43
Field C	CSA	–	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.
	CSA-Pi9	BC₂F₇	141	91	n.d.	n.d.	n.d.	n.d.	n.d.	1.86 ± 0.04	18.56

¹Field A: normal irrigation condition; Field B: normal irrigation condition in reclaimed region; Field C: high salinity condition in reclaimed region. Field locations are described in the “Materials and Methods” section
²Data represent the mean of five measurements. DAT: days after transplanting; n.a.: not applicable (all plants dead)
³Data represent mean ± standard error (SE; n = 5). Asterisks indicate statistically significant differences between CSA and CSA-Pi9 in each field (*P < 0.05, **P < 0.01, ***P < 0.001; Student's t-test). n.a.: not applicable (all plants died); n.d.: no data
⁴All values in this column represent grain yield per plant, estimated in grams (mean ± SE; n = 5; *P < 0.05; Student's t-test), except CSA-Pi9 in field C, which was harvested in bulk and total grain weight (334 g) was divided into the number of bulked plant (18 plants). This is the reason that there is no standard error in value of CSA-Pi9 in Field C