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Fig. 4 | Rice

Fig. 4

From: OsWRKY62 and OsWRKY76 Interact with Importin α1s for Negative Regulation of Defensive Responses in Rice Nucleus

Fig. 4

OsIMα1a and OsIMα1b were positively involved in resistance against rice blast pathogen. a The transgenic and wild-type (ZH17) plants of about 3-month-old were inoculated with a virulent strain of M. oryzae SZ by infiltration. The symptoms were photographed 10 days after the challenge. Bar = 2 cm. b Lesion length was measured from the inoculated leaves. P-values were calculated by one-tailed Student's t-test, n ≥ 8. The experiment was repeated twice with similar results. cj Gene expression was determined by qRT-PCR analyses using rice ubiquitin gene as an internal standard. Values represent means ± SD (n = 3). Two independent experiments were performed and obtained similar results. CDU for overexpression of OsIMα1a and OsIMα1b, KO for knockout, and imα1abKO for double knockout mutants of OsIMα1a and OsIMα1b. Significant differences (Student's t-test) compared with the wild-type plants are listed in the figure (P-value < 0.05, *; < 0.01, **; < 0.001,***)

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