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Rice
Fig. 4 | Rice

Fig. 4

From: Rice Carbohydrate-Binding Malectin-Like Protein, OsCBM1, Contributes to Drought-Stress Tolerance by Participating in NADPH Oxidase-Mediated ROS Production

Fig. 4

OsCBM1 interacts with OsRbohA and showed very high coexpressions with OsRbohA. A Expression levels of OsCBM1 in the WT and osrbohA mutant according to a semi-quantitative RT-PCR experiment. B Expression levels of OsCBM1 in the WT and osrbohA mutant by qRT-PCR experiment with OsActin1 as the internal control. The leaves from 6-week-old plants under both control and drought were used for the determination. Data are means of three biological replicates (n = 12). Bars annotated with different letters represent values that are significantly different (p ≤ 0.05) according to a one-way ANOVA analysis. C Firefly luciferase complementation imaging (LCI) assay, showing the interaction of OsCBM1 with OsRbohA in vivo. D Bimolecular fluorescence complementation (BiFC) assay, showing the interaction of OsCBM1 with OsRbohA in vivo. E MBP-pull down assay, showing the interaction of OsCBM1 with OsRbohA-ND (coding 360 amino acid residues at the N-terminal) in vitro

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