Fig. 3
OsCBM1 positively regulates drought tolerance and reactive oxygen species (ROS) production of rice. A Phenotypes of wild-type (WT), OsCBM1-overexpressing (OE), and RNA interference (RNAi) plants before drought treatment (the top pictures), after drought (the middle pictures, without watering for 5 d), and re-watering for 14 d (the bottom pictures). Four-week-old plants were used for drought treatments. B Survival rates of the different plant lines after the drought. At least 50 plants were used in each survival statistics experiment. Data are means ± SD from three independent biological replicates. C The numbers of stomatal apparatuses in the abaxial epidermis of flag leaves between the different plant lines under normal growth conditions. At least 2000 stomata in 30 microscopy pictures from different leaves were used to calculate values for each line of plants. D Percentage of various stomatal apertures in the abaxial epidermis of flag leaves between the different plant lines. For each type of plant, at least 500 stomata apparatuses from different plant leaves were calculated. E Visualization of ROS levels in the protoplasts isolated from the various plant lines. Cytoplasmic ROS were detected with H2DCF-DA fluorescence. Bars = 50 μm. F H2O2 and O2− histochemical analyses in leaves of the 6-week-old plants under normal growth conditions stained with 1% 3,3′-diaminobenzidine tetrachloride (DAB) or 0.1% nitroblue tetrazolium (NBT), respectively. G, Contents of H2O2 (μmol g−1 DW), and production rate of O2− (nmol min−1 mg−1 protein) in leaves of the 6-week-old plants under normal growth and drought stress conditions. Bars annotated with different letters represent values that are significantly different (p ≤ 0.05) according to a one-way ANOVA