Fig. 4
From: Identification of Heterotic Loci with Desirable Allelic Interaction to Increase Yield in Rice
Validation of the Ghd8 effect on mid-parent heterosis (MPH) using near-isogenic lines (NILs) and complementation transgenic lines. a Graphical genotype of NIL-Ghd8NIP showing a single introduced Nipponbare segment encompassing Ghd8 in the ZS97 background. b The heterosis advantage of Ghd8 for the yield traits in hybrids of NIL-Ghd8NIP and NIL-Ghd8ZS97 in a two-year trial. Error bar represents the mean ± SE (n = 3). c The dominance degree of Ghd8 effect on spikelet number (SN) and grain yield per plant (YD) in hybrids. If the effect has an excessive degree of dominance, it is set to a score of 5.0. d Phenotypes of the complementation transgenic lines and their hybrids at maturity. Ghd8+, homozygous-positive transgenic line containing the alleles Ghd8NIP; Ghd8−, homozygous-negative control lines; F1 denotes the hybrid derived from the cross of the independent complementation transgenic plant and corresponding negative plant. Scale bars, 10 cm. e, f MPH of Ghd8 for SN (E) and YD (F) in the F1 hybrids across a two-year trial. The error bar represents the mean ± SE (n = 3)