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Fig. 5 | Rice

Fig. 5

From: Rice Brittle Culm19 Encoding Cellulose Synthase Subunit CESA4 Causes Dominant Brittle Phenotype But has No Distinct Influence on Growth and Grain Yield

Fig. 5

Functional identification of Bc19. a PCR assay of transgenic lines of brittle gene Bc19. M, DL-2000 plus marker; WT, the wild-type parent Nipponbare (the transgenic acceptor, as PCR-negative control); CK, pC2300-Bc19 plasmid (PCR-positive control); TG1 and TG2, positive transgenic lines. b and c Comparison of cell wall components among the wild type plant, mutant Bc19 and two positive transgenic lines, in leaves and culms, respectively. d Transcript levels of OsCESA4 in different tissues of the wild type (WT), the mutant Bc19, the hybrid F1 plants from a cross between WT and Bc19, and two transgenic lines (TG1 and TG2), respectively. SL, seedling leaves; SR, seedling roots; L and C, leaves and culms from plants two weeks after heading, respectively; P, young spikelets. The expression data of WT were all set to 1.0 and those of other plants were adjusted accordingly. Bars represent standard deviations of three independent experiments. Double asterisks signify statistically significant differences compared to WT at p < 0.01

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