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Fig. 1 | Rice

Fig. 1

From: The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice

Fig. 1

Pleiotropic effects of the mutants of OsSPL4 in Nipponbare background. A Gene structure of OsSPL4 and the sequence alignment between the mutants in T3 generation and control plants. Nip, Nipponbare. The deleted amino acid residues in the mutants are presented by dashes. The protospacer adjacent motif (PAM) are underlined in red. Two types of mutation events (spl4-d3 and spl4-d15) were generated by CRISPR/Cas9 in Nipponbare rice. B The two different mutation events were confirmed by Sanger sequencing. CH Comparisons of several important traits among spl4-d3, spl4-d15 lines and control plants. C Flag leaf, scale bar = 10 cm. D Panicle, scale bar = 5 cm. E Stem, scale bar = 10 cm. F Plant height. G Flag leaf length. H Flag leaf width. I Panicle length. P values were determined by Student’s t test. *P < 0.05, **P < 0.01

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