Fig. 2

Expression patterns of OsLCT2. a–c The expression of OsLCT2 was determined by qRT-PCR. a Tissue-dependent expression of OsLCT2 in 2-week-old seedlings. The leaf, root and basal stem (2 cm above the roots) were sampled for expression analysis. Relative expression of OsLCT2 is shown, with its expression in the basal stem set to 1. b Response of OsLCT2 in seedling roots to the absence or excess of Fe, Mn or Zn. Seven-day-old seedlings were grown in Yoshida nutrient solution without Fe, Mn, or Zn or with high concentrations of Fe, Mn, or Zn for 7 d. Seedlings grown in standard Yoshida nutrient solution were used as controls. c Response of OsLCT2 in seedling roots to external Cd. Two-week-old seedlings were exposed to different concentrations of Cd for 24 h. Data show means ± SD of three biological replicates. In b and c, asterisks above the bars indicate significant differences between the treatments and controls (*P < 0.05, **P < 0.01; Student’s t-test). d Histochemical GUS staining of roots of transgenic rice plants transformed with OsLCT2 promoter-driven GUS constructs. Tissue-specific expression of OsLCT2 in the entire roots (I), root tip (II), lateral roots (III), root elongation zone (IV, V), and maturation zone (VI, VII) visualized in cross section (IV, VI) and vertical section (V, VII)