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Fig. 6 | Rice

Fig. 6

From: Rice miR1432 Fine-Tunes the Balance of Yield and Blast Disease Resistance via Different Modules

Fig. 6

OsACOT is not involved in the regulation of rice blast resistance. a The relative mRNA levels of OsACOT in OX1432, MIM1432, and the Nipponbare control (NPB). b The relative mRNA levels of OsACOT in the transgenic lines overexpressing OsACOT (OXACOT) and the Nipponbare control. For a and b, total RNA was extracted from three to five-leaf stage seedlings for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. c Blast disease phenotypes of OXACOT and the Nipponbare control at 5 days post-inoculation (dpi) of indicated M. oryzae strains. Bar = 5 mm. d Quantification analysis of the relative fungal biomass in c. The relative fungal biomass was measured by using the ratio of DNA level of M. oryzae MoPot2 genes against that of rice ubiquitin. For a, b, and d, error bars indicate SD (n = 3 independent samples). N.D. indicates no difference as determined by a one-way ANOVA analysis. All the experiments were repeated two times with similar results

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