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Fig. 4 | Rice

Fig. 4

From: Rice miR1432 Fine-Tunes the Balance of Yield and Blast Disease Resistance via Different Modules

Fig. 4

OsEFH1 enhances rice blast disease resistance. a The relative mRNA levels of OsEFH1 in transgenic lines overexpressing OsEFH1 (OXEFH1) and the Nipponbare (NPB) control. Total RNA was extracted from three to five-leaf stage seedlings for reverse-transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. The relative mRNA levels of OXEFH1 lines were normalized with that of the Nipponbare control. b The blast disease phenotypes of OXEFH1 lines and the Nipponbare control at 5 days post-inoculation (dpi) of indicated M. oryzae strains. Bar = 5 mm. c The Quantification analysis of the relative fungal biomass in b. The fungal biomass was shown as the ratio of DNA level of M. oryzae MoPot2 genes against that of rice ubiquitin. d Quantification analysis of the fungal development during the invasive process. Over 200 conidia in each line were analyzed. For a and c, error bars indicate SD (n = 3 independent samples). Different letters above the bars indicate a significant difference (P < 0.01) as determined by a one-way Tukey–Kramer analysis. All the experiments were repeated two times with similar results

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