Fig. 1
From: Rice miR1432 Fine-Tunes the Balance of Yield and Blast Disease Resistance via Different Modules
Overexpression of miR1432 enhances rice susceptibility to Magnaporthe oryzae. a The relative amount of mature miR1432 in the transgenic lines overexpressing MIR1432 gene (OX1432) and the Nipponbare (NPB) control. Reverse-transcription (RT) was carried out with total RNA and a miR1432 specific stem-loop RT primer (Additional file 2: Table S2). The RT product was subsequently used as a template for quantitative polymerase chain reaction (q-PCR) to detect the amounts of miR1432. The amounts of snRNA U6 were examined and used as an internal reference. b The blast disease phenotypes on leaves 5 days post-inoculation of M. oryzae strains GZ8, RB22, and DZ96, respectively. Bar = 5 mm. c The relative fungal biomass of the indicated strains in OX1432 and the Nipponbare control. The fungal biomass was determined by using the ratio of DNA levels of M. oryzae MoPot2 against the DNA levels of rice ubiquitin. Error bars indicate SD (n = 3 independent samples). Different letters above the bars indicate significant differences (P < 0.01) as determined by One-way Tukey–Kramer analysis. Similar results were obtained in at least two independent experiments