Fig. 7

OsFLZ18 negatively regulates early seedling growth in air and coleoptile elongation under submergence stress. a Relative expression of OsFLZ18 in WT and three OE transgenic lines by qRT-PCR. b Protein accumulations of OsFLZ18-GFP in WT and three OE transgenic lines by Western Blotting. Total protein was extracted from seedlings, and antibodies against GFP and Histone 3 were used in this assay. c qRT-PCR analysis of the transcriptional levels of SnRK1A and αAmy3 in WT and OE transgenic lines. *p < 0.05, **p < 0.01, Student’s t-test. d Phenotypes of WT and OE transgenic lines in air and under submerged conditions. Sterile seeds were germinated for 7 days in air and under complete submergence. Bars = 1 cm. e, f Statistical data for the shoot lengths (e) or coleoptile lengths (f) of WT and OE transgenic plants shown in (d) (n > 30). **p < 0.01, Student’s t-test