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Fig. 6 | Rice

Fig. 6

From: Genome-Wide Identification, Expression and Functional Analysis Reveal the Involvement of FCS-Like Zinc Finger Gene Family in Submergence Response in Rice

Fig. 6

OsFLZ18 physically interacts with and antagonizes the SnRK1A-dependent transcriptional activation to αAmy3. a Pull-down assay confirmed the interaction between OsFLZ18 and SnRK1A. b BiFC assay showed the interaction between OsFLZ18 and SnRK1A in Nicotiana benthamiana leaves. Scale bars: 50 μm. c Schematic constructs of the reporters and effectors used in the dual-luciferase reporter assay. d Dual-luciferase reporter assay showing the inhibition effect of OsFLZ18 on the transcriptional activation of αAmy3 by SnRK1A. Data are means ± SD from four biological replicates. NS no significant, **p < 0.01, Student’s t test

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