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Rice
Fig. 7 | Rice

Fig. 7

From: Global Survey of Alternative Splicing in Rice by Direct RNA Sequencing During Reproductive Development: Landscape and Genetic Regulation

Fig. 7

Validation the transcriptional inhibition role of alternative spliced transcription factors. a Coexpression of Colike and Colike.2 with LUC driven by the CKI promoter in protoplasts. Empty vector of pGREENII 0800-LUC mixed with the relative isoform was used as negative control. b Coexpression of MADS2 and MADS2.2 with LUC driven by the DL promoter in protoplasts. Empty vector of pGREENII 0800-LUC mixed with the relative isoform was used as negative control. c Coexpression of MADS2 and MADS2.2 with LUC driven by the MADS16 promoter in protoplasts. Empty vector of pGREENII-62-SK mixed with the relative promoter of the targets were used as control. Bar graphs show means with two biological replications. Error bars show ± SD. The relative luminescence of control was set as 1.0

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