Fig. 2

Validation of different alternative splicing (AS) events by RT-PCR. Fourteen AS isoforms were selected to check each isoform in RT-PCR and eleven AS were detectable at the mRNA level either with changed transcript levels or new isoforms. The primer of each hand used for variation were arrowed and the primer position numbered from transcription start sites ATG were indicated. The product size of each alternative isoform was showed. The cycles of RT-PCR were showed in Additional file 2: Table S3. The dRNA-seq data of the above DAS events were showed in Additional file 1: Figure S3. Actin2 was used as the inner control