Fig. 4

LPA1 and KLP directly activate PIN1a. a Schematic diagram showing the location of the probes (P1-P3 and P4-P6) used for chromatin immunoprecipitation (ChIP) assay within the 1.5 kb promoter regions of PIN1a and PIN3b, respectively. b The DNA fragments were immunoprecipitated from p35S:KLP:GFP transgenic plants calli, and the enrichment was analyzed by qPCR. Input DNA was used to normalize the data. Anti-GFP antibody was used for immunoprecipitation with pre-immune serum as control. Error bars represent the mean ± SE (n = 3). Different letters indicate significant differences at P < 0.05. c Schematic diagram indicating the constructs used in the transient assay. 1.5 kb of PIN1a promoter was used to drive ß-glucuronidase (GUS) gene coding sequences. 35S promoter was used to drive LPA1, KLP or luciferase (Luc) gene OFR sequences. d Plasmids corresponding to p35S:LPA1, p35S:KLP, p35S:KLP + p35S:LPA1 were co-transformed with the vector expressing the GUS under the control of the PIN1a promoter (pPIN1a) in protoplasts. The luciferase expression level was utilized to normalize the GUS expression. Error bars represent the mean ± SE (n = 3). Different letters indicate significant differences at P < 0.05