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Rice
Fig. 1 | Rice

Fig. 1

From: Loose Plant Architecture 1-Interacting Kinesin-like Protein KLP Promotes Rice Resistance to Sheath Blight Disease

Fig. 1

Interaction between KLP and LPA1. a The interaction between LPA1 and KLP or IDD13 was analyzed by yeast two hybrid (Y2H) assay. BD: GAL4-DNA binding domain; AD: activation domain; −TL: SD medium without tryptophan and leucine; −TLH: SD medium without tryptophan, leucine, and histidine. b LPA1-nYFP + − cYFP or LPA1-nYFP + KLP-cYFP were coexpressed in rice protoplasts to detect YFP protein reconstruction. Bars = 10 μm. c The interaction between LPA1 and KLP was analyzed in tobacco leaves by co-IP. LPA1-GFP+ KLP-Myc or LPA1-GFP were transformed into tobacco leaves using Agrobacterium-mediated transformation. Western blot analysis used an anti-Myc or anti-GFP antibody. Anti-GFP antibody was used to immunoprecipitation. d Relative expression patterns of LPA1 and KLP were examined at 0, 24, 48, and 72 h post-inoculation (hpi) with R. solani AG1-IA. The error bars indicate the mean ± SE (n = 3). Different letters indicate significant differences at P < 0.05

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