Proteomic and Transcriptomic Analyses Provide Novel Insights into the Crucial Roles of Host-Induced Carbohydrate Metabolism Enzymes in Xanthomonas oryzae pv. oryzae Virulence and Rice-Xoo Interaction

Rice

Table 1 Proteins exhibited significantly different expression patterns (|fold change| > 1.5) in X. oryzae pv. oryzae at all three time points after susceptible rice leaf extract (RLX) treatment

Protein name^a	Protein code^a	Accession no.^a	3 h Fold change (+RLX/−RLX)^b	6 h Fold change (+RLX/−RLX)^b	12 h Fold change (+RLX/−RLX)^b	Function/Similarity^c	Functional catalog^c	Predicted cellular localization^d	pI (cal)^e	M_w (cal) kDa^e
XanA	PXO_03174	gi\|188,523,095	+ 2.77	+ 1.60	+ 2.76	Phosphohexose mutase	Carbohydrate transport and metabolism	Cytoplasmic; Periplasmic	5.19	49.13
RocF	PXO_02850	gi\|188,523,421	+ 3.74	+ 3.22	+ 8.85	Arginase	Amino acid transport and metabolism	Cytoplasmic	5.28	33.36
Imp	PXO_00388	gi\|188,520,635	+ 1,000,000	+ 3.37	+ 1.69	Inositol-1-monophosphatase	Carbohydrate transport and metabolism	Cytoplasmic	7.62	30.27
Bfr	PXO_01151	gi\|188,577,142	−2.24	−1.63	−1.71	Bacterioferritin	Inorganic ion transport and metabolism	Cytoplasmic	4.93	18.73
MinD	PXO_04464	gi\|188,519,996	−1.69	−1.96	−1.88	Septum site-determining protein MinD	Cell cycle control, cell division, chromosome partitioning	Cytoplasmic; innermembrane	5.32	28.93

^aThe protein name, protein code and accession number of identified proteins were according to genomic annotation of X. oryzae pv. oryzae PXO99^A
^bThe average fold change in NB medium plus rice leaf extract (+RLX) compared to NB medium (−RLX)
^cThe similarity and functional catalog were performed by using protein blast (http://blast.ncbi.nlm.nih.gov/Blast.cgi) and eggNOG 4.5 (http://eggnogdb.embl.de/)
^dBacterial protein subcellular localization prediction was performed by PSORTb v.3.0 (http://www.psort.org/psortb/)
^eComputation of the theoretical pI (isoelectric point) and Mw (molecular weight) for identified proteins was performed by expasy tool (http://web.expasy.org/compute_pi/)