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Rice
Fig. 3 | Rice

Fig. 3

From: Proteomic and Transcriptomic Analyses Provide Novel Insights into the Crucial Roles of Host-Induced Carbohydrate Metabolism Enzymes in Xanthomonas oryzae pv. oryzae Virulence and Rice-Xoo Interaction

Fig. 3

Detection and assessment of cellular defense responses in rice IR24 at 1 and 3 d after infection with PXO99A, ΔxanA or Δimp. A DAB staining and visualization of hydrogen peroxide in IR24 rice. 5-week-old rice leaves were detached and infiltrated with DAB staining solution (1 mg/mL, pH 3.8) used for oxidative burst detection after inoculation with H2O, PXO99A, ΔxanA and Δimp via 1-ml needleless syringes. PXO99A-inoculated leaves were used as controls. Orange-brown deposits were visualized after the leaves were cleared in absolute ethanol. Representative photos were taken at 1 and 3 dpi. B Quantification of H2O2 levels in rice leaves after inoculation with H2O, PXO99A, ΔxanA and Δimp via 1-ml needleless syringes. Values are the means and standard deviation of three independent experiments, each of which comprised two replicates. Asterisks indicate significant differences compared with controls (t test, ** indicates P < 0.01)

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