Fig. 4
Variations in temporal expression patterns of various glutelin mRNAs in 9311-2x and 9311-4x (harvested in 2019). qRT-PCR analyses of glutelin gene expressions (GluA-1, GluA-2, GluB-1, GluB-2, GluC-1, and GluD-1) in developing seeds of 9311-2x and 9311-4x. The y-axis represents glutelin mRNA expression level relative to the β-actin mRNA level. The x-axis represents the day of seed collection after flowering. Significant differences in glutelin gene transcript levels between 9311-2x and 9311-4x at the same developmental stage were tested using independent Student’s t-test (∗p < 0.05, ∗∗p < 0.01). Data are presented as means ± standard errors of three biological replicates