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Fig. 5 | Rice

Fig. 5

From: Comparisons within the Rice GA 2-Oxidase Gene Family Revealed Three Dominant Paralogs and a Functional Attenuated Gene that Led to the Identification of Four Amino Acid Variants Associated with GA Deactivation Capability

Fig. 5

Two functionally distinct clades of class I GA2ox genes in monocots according to phylogenetic analysis and their functional identifications. a Phylogenetic analysis of class I GA2ox genes in monocots revealed two functionally distinct clades. b Functional identification of orthologous genes of Bd2g50280 (orthologous to OsGA2ox3), Bd2g19900 (orthologous to OsGA2ox4), Bd2g06670 (orthologous to OsGA2ox7), and Bd2g32577 (orthologous to OsGA2ox10) from B. distachyon. Three representative T0 transgenic rice plants from each of the transgenic overexpression lines and their average RPH are shown. Values are means ± SE (n = 5). The RT-PCR analysis results for each target gene of three independent transgenic lines are shown. The Actin gene was used as a control. c Statistical comparisons of RPH between clades A and B as a result of the orthologous genes from B. distachyon and rice. Values are means ± SE (n = 5). The different letters indicate significant differences between samples according to the Turkey’s HSD test (p < 0.05)

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