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Rice
Fig. 4 | Rice

Fig. 4

From: Comparisons within the Rice GA 2-Oxidase Gene Family Revealed Three Dominant Paralogs and a Functional Attenuated Gene that Led to the Identification of Four Amino Acid Variants Associated with GA Deactivation Capability

Fig. 4

Phenotypic comparison of the transgenic rice plants overexpressing all the rice GA2ox genes and characterization of the OsGA2ox7-OX transgenic lines. a Phenotypic comparison of the transgenic rice plants overexpressing for all rice GA2ox genes. A representative T0 transgenic plant overexpressing each of the 9 rice GA2ox genes under the same comparison basis is shown. The plants were grown in a greenhouse. b Phenotypic comparison of the OsGA2ox7-OX overexpression transgenic lines. The insertion events of lines 1 and 3 were identified, and their phenotypes vs genotypes (W/W no T-DNA; T/W hetero; T/T homo) and RPH compared with that of the NT plants are shown. The plants were grown in paddy fields. c Southern blot assay showing a single T-DNA insertion for all three lines. The line-1 and line-3 showing independent insertion events. d Results of RT-PCR analysis of various genotypes of OsGA2ox7-OX transgenic line-1 and line-3. The Actin gene was used as a control

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