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Rice
Fig. 2 | Rice

Fig. 2

From: Comparisons within the Rice GA 2-Oxidase Gene Family Revealed Three Dominant Paralogs and a Functional Attenuated Gene that Led to the Identification of Four Amino Acid Variants Associated with GA Deactivation Capability

Fig. 2

Southern blot assay of mutant M43852 and a schematic illustration of the RT-PCR results and possible cDNA and exon/intron structures to reannotate the gene structure of OsGA2ox2. a Southern blot assay showing only one copy of the T-DNA insertion in mutant M43852. b A schematic diagram showing five genes (named gene-01 to gene-05) annotated in a 40 kb region flanking the T-DNA insertion site. c Enlarged regions of annotated gene-03 (LOC_Os01g22920) and gene-04 (LOC_Os01g22910) with their possible exon (box) and intron (bold line) structures. The two primer sets (arrows) utilized to detect gene expression are shown above their genes. d cDNAs and expected sizes of RT-PCR products using the corresponding primer sets for the annotated gene-03, gene-04 and reannotated gene OsGA2ox2. e RT-PCR results of gene-03 (using the red primer set), gene-04 (using the yellow primer set that amplify 592 bp) and the reannotated gene OsGA2ox2 (using the 5′ red and 3′ yellow primer sets that amplify 868 bp). f Accurate cDNAs for the reannotated OsGA2ox2. Three exons with 370 amino acids and the relative locations of the DIOX_N and 2OG-FeII_Oxy conserved protein domains

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