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Rice
Fig. 11 | Rice

Fig. 11

From: Oryza sativa ObgC1 Acts as a Key Regulator of DNA Replication and Ribosome Biogenesis in Chloroplast Nucleoids

Fig. 11

Analysis of polysome fractions in wild-type and obgc1-d1 mutant plants. Total ribosomes from leaves of 10-day-old wild-type (Dongjin) and obgc1-d1 mutant plants were separated by a sedimentation ultracentrifugation in a 15–55% sucrose gradient. Nine fractions (1–9) of equal volume were collected from top to bottom. RNAs from each fraction were separated on 1.2% agarose gels, transferred to nylon membranes, and hybridized with probes prepared from gene-specific sequences for 16S/23S rRNAs. Cytosolic 17S rRNA (17S) was used as a loading or negative control

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