Fig. 3

Subcellular localization and expression analysis of PSL50. a GFP signals in rice protoplasts. NST1-mCherry was used as a rice protoplast Golgi-tagged marker. b PSL50 expression in different parts of a flag leaf. Different letters indicate significant differences according to one-way ANOVA and Duncan’s test (P < 0.05). Data are mean ± SD (n = 3). c Relative expression levels of PSL50 in various organs at different growth stages. Data are presented as mean ± SD (n = 3). d Expression analysis of PSL50 in hydroponic WT and psl50 seedlings under heat stress conditions. Data are mean ± SD (n = 3). e Expression analysis of ROS scavenging system-related genes in hydroponic WT and psl50 seedlings at 26 °C (upper) and 45 °C (lower) for 12 h. Data are mean ± SD (n = 3). Rice UBIQUITIN (LOC_Os03g13170) was used as an internal control. f Levels of thylakoid membrane proteins detected in total proteins from top second leaves of 12 d WT and psl50 hydroponic seedlings at 26 °C and 45 °C for 50 h. Image J was used for the quantification of immunoblot results and these experiments were repeated at least twice with similar results. g Leaf chlorophyll content of hydroponic WT and psl50 seedlings before and after heat treatment. Data are means ± SD (n = 3). h-j Changes of photochemical efficiency of PSII (F_v/F_m), non-photochemical fluorescence quenching (NPQ) and relative PSII electron transport rate (ETR) in WT and psl50 under heat treatment. The data for heat treatment in g-h were collected after a 7-d recovery at 26 °C. Seedlings were hydroponically grown at 26 °C for 12 d and then treated at 45 °C for 50 h following a 7-d recovery at 26 °C. CK, normal growth condition at 26 °C; HT, heat treatment at 45 °C followed by recovery at 26 °C for 7 d. Data are means ± SD (n = 5). Asterisks indicate significant difference by Student’s t test (**P < 0.01 and *P < 0.05)