Fig. 8

OsGSAM protein truncation experiment. a Schematic diagram of OsGSAM protein truncation. FL represents the full-length OsGSAM, and R1-R10 correspond to truncated fragments containing the 1st-240th, 241th–478th, 81th–400th, 161th–320th, 1st-160th, 321th–478th, 1st-80th, 81th–160th, 321th–400th and 401th–478th amino acid residues of OsGSAM, respectively. The red arrow indicates the substitution of threonine for alanine at the 171th amino acid residue of OsGSAM in the ys53 mutant. b The yeast two-hybrid experiment of the full-length OsGSAM with itself. c Yeast two-hybrid experiments between the R1, R2, R3 and R4 fragments and the full-length GSAM. d Yeast two-hybrid experiments between the R5, R6, R7, R8, R9 and R10 fragments and the full-length GSAM. The FL was combined with empty pGADT7 vector as internal control. Interaction between pGADT7-T and pGBKT7-Lam was used as a negative control, and interaction between pGADT7-T and pGBKT7–53 was used as a positive control. Co-transformed yeast colonies were spotted on the selective SD medium minus Trp and Leu (−LT), the normally surviving monoclonal bacteria were selected and then dripped to the SD medium minus His, Leu, Trp and Ade to grow (−AHLT). 1X, 10X, and 100X represent the saturated culture solution of transformant diluted to concentrations of 1, 0.1 and 0.01, respectively